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KMID : 0380619940260050633
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Korean Journal of Food Science and Technology
1994 Volume.26 No. 5 p.633 ~ p.637
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Characterization of Streptomyces sp . KSM - 35 and Purification of Its Maltotetraose Forming Amylase
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Abstract
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A bacterial strain KSM-35 producing maltotetraose forming amylase was isolated from compost and identified as Streptomyces based on its morphological, cultural, and physiological characteristics. The amylase from Streptomyces sp. KSM-35 culture filtrate was purified by ammonium sulfate precipitation, followed by the liquid chromatographic procedures using DEAE-Toyo pearl and sephadex G-100 with 27.1% activity recovery. The rpolecular weight of the enzyme was estimated to be 50,000 and the isoelectric point 4.3. The main product by the amylase from soluble starch was maltotetraose which accounted for 56% of all the oligosaccharides detected after 26 hrs of reaction. Maltose (20%o) and maltotriose (16%) were the next important byproducts while glucose and maltopentaose were detected as traces.
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